phone icon
+44 (0)1223 298875

Anti-PKC Zeta/Lambda Antibody

file icon Datasheet
ABclonal
A0618
Overview
Name: Anti-PKC Zeta/Lambda Antibody
Description: Rabbit polyclonal antibody to PKC zeta/lambda.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200
Reactivity: Human
Immunogen: A synthetic peptide of human PRKCI
Protein Length: 596
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis.
Tissue Specificity: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Post-Translational Modification: Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334. Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
Cellular Location: Cytoplasm. Membrane. Endosome. Nucleus.

Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A.
UniProt: P41743
Gene ID: 5584

Images
Western Blot - Anti-PKCzeta/lambda Antibody from ABclonal (A0618) - Antibody Supply Service
Western blot analysis of extracts of MCF7 cells tissue, using PRKCI antibody.

Product Code - A0618
Size Price Quantity
100µl £243
200µl £372

or
Request a quotation
Contact us directly to order
  • call icon +44 (0)1223 298875
  • +44 (0)1223 281447
  • email icon orders@ab-y-ss.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe