|Description:||Rabbit polyclonal antibody to TFII-I.|
|Applications:||WB, IHC, IF, FC|
|Immunogen:||A synthetic peptide of human GTF2I|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||BAP-135; BAP135; BTKAP1; DIWS; FLJ38776; FLJ56355; IB291; SPIN; TFII-I; WBS; WBSCR6|
|Background:||The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:200 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100, FC 1:20 - 1:50|
Target (Information from UniProt)
|Function:||Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.|
|Tissue Specificity:||Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.|
|Sequence Similarities:||Belongs to the TFII-I family.|
|Post-Translational Modification:||Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.|
|Cellular Location:||Cytoplasm. Nucleus.
Colocalizes with BTK in the cytoplasm.
Western blot analysis of extracts of 293 cells tissue, using GTF2I antibody.