phone icon
+44 (0)1223 298875

Anti-TFII-I Antibody

file icon Datasheet
ABclonal
A0617
Details
Description: Rabbit polyclonal antibody to TFII-I.
Category: Primary Antibodies
Applications: WB, IHC, IF, FC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P78347
Gene ID: 2969
Immunogen: A synthetic peptide of human GTF2I
Protein Length: 998
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: BAP-135; BAP135; BTKAP1; DIWS; FLJ38776; FLJ56355; IB291; SPIN; TFII-I; WBS; WBSCR6
Purification: Affinity purification
Background: The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:200 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100, FC 1:20 - 1:50

Target (Information from UniProt)
Function: Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Tissue Specificity: Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
Sequence Similarities: Belongs to the TFII-I family.
Post-Translational Modification: Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Cellular Location: Cytoplasm. Nucleus.

Colocalizes with BTK in the cytoplasm.

Images
Western Blot - Anti-TFII-I Antibody from ABclonal (A0617) - Antibody Supply Service
Western blot analysis of extracts of 293 cells tissue, using GTF2I antibody.

Product Code - A0617
Size Price Quantity
100µl £243
200µl £372

or
Request a quotation
Contact us directly to order
  • call icon +44 (0)1223 298875
  • +44 (0)1223 281447
  • email icon orders@ab-y-ss.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe