Anti-14-3-3 β/α AntibodyDatasheet
|Name:||Anti-14-3-3 β/α Antibody|
|Description:||Rabbit polyclonal antibody to 14-3-3 β/α.|
|Applications:||WB, IHC, IF|
|Dilutions:||WB 1:500 - 1:1000, IHC 1:50 - 1:100, IF 1:20 - 1:50|
|Immunogen:||A synthetic peptide of human YWHAB|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.|
|Sequence Similarities:||Belongs to the 14-3-3 family.|
|Post-Translational Modification:||The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.|
|Cellular Location:||Cytoplasm. Melanosome.
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Western blot analysis of extracts of Jurkat cells, using YWHAB antibody.