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Anti-RPA32 Antibody

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ABclonal
A0568
Overview
Name: Anti-RPA32 Antibody
Description: Mouse monoclonal antibody to RPA32.
Applications: WB
Dilutions: WB 1:500 - 1:1000, IHC 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human RPA2
Protein Length: 270
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Sequence Similarities: Belongs to the replication factor A protein 2 family.
Post-Translational Modification: Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.
Cellular Location: Nucleus. Nucleus > PML body.

Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.
UniProt: P15927
Gene ID: 6118
Synonyms: 60S acidic ribosomal protein P1 Antibody
AA409079 Antibody
AI325195 Antibody
AU020965 Antibody
ik:tdsubc_2g1 Antibody
M(2)21C Antibody
MGC137236 Antibody
OTTHUMP00000004008 Antibody
p32 Antibody
p34 Antibody
RCJMB04_6d17 replication protein A2, 32kDa Antibody
REPA2 Antibody
Replication factor A protein 2 Antibody
Replication protein A Antibody
Replication protein A 32 kDa subunit Antibody
Replication protein A 32kDa subunit Antibody
Replication protein A 34 kDa subunit Antibody
Replication protein A2 Antibody
replication protein A2 (32kD) Antibody
Replication Protein A2 (32kDa) Antibody
Replication protein A2, 32kDa Antibody
RF A protein 2 Antibody
RF-A protein 2 Antibody
Rf-A2 Antibody
RFA Antibody
RFA2_HUMAN Antibody
RP A p32 Antibody
RP-A p32 Antibody
RP-A p34 Antibody
RP21C Antibody
RPA Antibody
RPA 2 Antibody
RPA 32 Antibody
Rpa2 Antibody
RPA32/RPA2 Antibody
RPA32/RPA2 (phospho S33) Antibody
RPA32/RPA2 (phospho S4 + S8) Antibody
RPA32/RPA2 (phospho T21) Antibody
RPA34 Antibody
RpLP1 Antibody
RpP2 Antibody
xx:tdsubc_2g1 Antibody
zgc:109822 Antibody

Images
Western Blot - Anti-RPA32 Antibody from ABclonal (A0568) - Antibody Supply Service
Western blot analysis of extracts of HeLa cells tissue, using RPA2 antibody.

Product Code - A0568
Size Price Quantity
100µl £243
200µl £372

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