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Anti-NEDD4 Antibody

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ABclonal
A0552
Overview
Name: Anti-NEDD4 Antibody
Description: Rabbit polyclonal antibody to NEDD4.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200
Reactivity: Human
Immunogen: Recombinant protein of human NEDD4
Protein Length: 1319 (1247)
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).
Post-Translational Modification: Auto-ubiquitinated.
Cellular Location: Cytoplasm. Cell membrane.

Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
UniProt: P46934
Gene ID: 4734
Synonyms: Cell proliferation-inducing gene 53 protein Antibody
E3 ubiquitin protein ligase Nedd4 Antibody
E3 ubiquitin-protein ligase NEDD4 Antibody
KIAA0093 Antibody
NEDD 4 Antibody
NEDD-4 Antibody
NEDD4_HUMAN Antibody
Neural precursor cell expressed developmentally down regulated 4 Antibody
Neural precursor cell expressed developmentally down-regulated protein 4 Antibody

Images
Western Blot - Anti-NEDD4 Antibody from ABclonal (A0552) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using NEDD4 antibody.
Immunohistochemistry - Anti-NEDD4 Antibody from ABclonal (A0552) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human prostate using NEDD4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - Anti-NEDD4 Antibody from ABclonal (A0552) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human gastric using NEDD4 antibody at dilution of 1:200 (40x lens).

Product Code - A0552
Size Price Quantity
50µl £148
100µl £243
200µl £372

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