|Description:||Rabbit polyclonal antibody to DDX58.|
|Applications:||WB, IHC, IF|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:100|
|Immunogen:||A synthetic peptide of human DDX58|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.|
|Tissue Specificity:||Present in vascular smooth cells (at protein level).|
|Involvement in Disease:||Singleton-Merten syndrome 2: A form of Singleton-Merten syndrome, an autosomal dominant disorder characterized by marked aortic calcification, dental anomalies, osteopenia, acro-osteolysis, and to a lesser extend glaucoma, psoriasis, muscle weakness, and joint laxity. Additional clinical manifestations include particular facial characteristics and abnormal joint and muscle ligaments. SGMRT2 is an atypical form characterized by variable expression of glaucoma, aortic calcification, and skeletal abnormalities, without dental anomalies.|
|Sequence Similarities:||Belongs to the helicase family. RLR subfamily.|
|Post-Translational Modification:||Phosphorylated in resting cells and dephosphorylated in RNA virus-infected cells. Phosphorylation at Thr-770, Ser-854 and Ser-855 results in inhibition of its activity while dephosphorylation at these sites results in its activation.|
|Cellular Location:||Cytoplasm. Cell projection > Ruffle membrane. Cytoplasm > Cytoskeleton. Cell junction > Tight junction.
Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles.
DEAD (Asp Glu Ala Asp) box polypeptide 58 Antibody
DEAD (Asp Glu Ala Asp/His) box polypeptide Antibody
DEAD box protein 58 Antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 Antibody
Probable ATP dependent RNA helicase DDX58 Antibody
Probable ATP-dependent RNA helicase DDX58 Antibody
Retinoic acid inducible gene 1 protein Antibody
Retinoic acid-inducible gene 1 protein Antibody
Retinoic acid-inducible gene I protein Antibody
RIG I Antibody
RLR 1 Antibody
RNA helicase Antibody
RNA helicase RIG I Antibody
Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using DDX58 antibody. Blue: DAPI for nuclear staining.