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Anti-CDC25B Antibody

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ABclonal
A0534
Details
Description: Rabbit polyclonal antibody to CDC25B.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P30305
Gene ID: 994
Immunogen: Recombinant protein of human CDC25B
Protein Length: 580
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Background: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000

Target (Information from UniProt)
Function: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Sequence Similarities: Belongs to the MPI phosphatase family.
Post-Translational Modification: Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Cellular Location: Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Cytoplasm > Cytoskeleton > Spindle pole.

Images
Western Blot - Anti-CDC25B Antibody from ABclonal (A0534) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using CDC25B antibody.

Product Code - A0534
Size Price Quantity
50µl £148
100µl £243
200µl £372

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