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Anti-FANCD2 Antibody

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ABclonal
A0521
Overview
Name: Anti-FANCD2 Antibody
Description: Rabbit polyclonal antibody to FANCD2.
Applications: WB
Dilutions: WB 1:500 - 1:1000
Reactivity: Human
Immunogen: A synthetic peptide of human FANCD2
Protein Length: 1471
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
Tissue Specificity: Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
Involvement in Disease: Fanconi anemia complementation group D2: A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
Post-Translational Modification: Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
Cellular Location: Nucleus.

Concentrates in nuclear foci during S phase and upon genotoxic stress. At the onset of mitosis, excluded from chromosomes and diffuses into the cytoplasm, returning to the nucleus at the end of cell division. Observed in a few spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites and could be sites of replication fork stalling. The foci are frequently interlinked through BLM-associated ultra-fine DNA bridges. Following aphidicolin treatment, targets chromatid gaps and breaks.
UniProt: Q9BXW9
Gene ID: 2177
Synonyms: DKFZp762A223 Antibody
FA 4 Antibody
FA D2 Antibody
FA4 Antibody
FAC D2 Antibody
FACD Antibody
FACD 2 Antibody
FACD2 Antibody
FACD2_HUMAN Antibody
FAD Antibody
FAD2 Antibody
FANC D2 Antibody
FANCD Antibody
FANCD 2 Antibody
FANCD2 (phospho S1404) Antibody
FANCD2 (phospho S222) Antibody
FANCONI ANEMIA COMPLEMENTATION GROUP D Antibody
Fanconi anemia complementation group D2 Antibody
Fanconi anemia group D2 protein Antibody
FANCONI PANCYTOPENIA TYPE 4 Antibody
FLJ23826 Antibody
OTTHUMP00000158853 Antibody
OTTHUMP00000207925 Antibody
Protein FACD2 Antibody
Type 4 Fanconi pancytopenia Antibody

Images
Western Blot - Anti-FANCD2 Antibody from ABclonal (A0521) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cells, using FANCD2 antibody.

Product Code - A0521
Size Price Quantity
100µl £243
200µl £372

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