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Anti-TrkB Antibody

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ABclonal
A0487
Overview
Name: Anti-TrkB Antibody
Description: Mouse monoclonal antibody to TrkB.
Applications: WB, FC
Dilutions: WB 1:500 - 1:1000, IHC 1:20 - 1:50, FC 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human NTRK2
Protein Length: 822(477)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
Tissue Specificity: Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.
Involvement in Disease: Obesity, hyperphagia, and developmental delay: A disorder characterized by early-onset obesity, hyperphagia, and severe developmental delay in motor function, speech, and language.
Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Post-Translational Modification: Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated.
Cellular Location: Cell membrane. Endosome membrane.

Internalized to endosomes upon ligand-binding.
UniProt: Q16620
Gene ID: 4915
Synonyms: AI848316 Antibody
BDNF tropomyosine receptor kinase B Antibody
BDNF/NT 3 growth factors receptor Antibody
BDNF/NT-3 growth factors receptor Antibody
Brain derived neurotrophic factor receptor Antibody
C030027L06Rik Antibody
EC 2.7.10.1 Antibody
gp140trk Antibody
GP145 TrkB Antibody
GP145-TrkB Antibody
GP145-TrkB/GP95-TrkB Antibody
GP145-TrkC Antibody
GP95 TrkB Antibody
High affinity nerve growth factor receptor Antibody
MTC Antibody
Neurotrophic tyrosine kinase receptor type 1 Antibody
Neurotrophic tyrosine kinase receptor type 2 Antibody
Neurotrophic tyrosine kinase receptor type 3 Antibody
Neurotrophin receptor tyrosine kinase type 2 Antibody
NT-3 growth factor receptor Antibody
NTRK 2 Antibody
NTRK1 Antibody
NTRK2 Antibody
NTRK2_HUMAN Antibody
NTRK3 Antibody
Obesity, hyperphagia, and developmental delay, included Antibody
p140-TrkA Antibody
RATTRKB1 Antibody
Tkrb Antibody
TRK Antibody
Trk B Antibody
Trk-A Antibody
Trk-B Antibody
Trk-C Antibody
TRK1-transforming tyrosine kinase protein Antibody
TRKA Antibody
TRKA+B Antibody
TrkA+B+C Antibody
TrkB (phospho Y515) Antibody
TrkB (phospho Y706 + Y707) Antibody
TrkB (phospho Y706) Antibody
TrkB tyrosine kinase Antibody
TRKB1 Antibody
TRKC Antibody
TrkC tyrosine kinase Antibody
Tropomyosin related kinase B Antibody
Tropomyosin-related kinase A Antibody
Tropomyosin-related kinase B Antibody
Tyrosine kinase receptor Antibody
Tyrosine kinase receptor A Antibody
Tyrosine kinase receptor B Antibody
Tyrosine receptor kinase B Antibody

Images
Western Blot - Anti-TrkB Antibody from ABclonal (A0487) - Antibody Supply Service
Western blot analysis of extracts of U251 cells, using AKT antibody.

Product Code - A0487
Size Price Quantity
100µl £243
200µl £372

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