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Anti-APEX1 Antibody

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ABclonal
A0476
Overview
Name: Anti-APEX1 Antibody
Description: Mouse monoclonal antibody to APEX1.
Applications: WB
Dilutions: WB 1:500 - 1:1000, IHC 1:20 - 1:50, IF 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human APEX1
Protein Length: 318
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
Sequence Similarities: Belongs to the DNA repair enzymes AP/ExoA family.
Post-Translational Modification: Phosphorylated. Phosphorylation by kinase PKC or casein kinase CK2 results in enhanced redox activity that stimulates binding of the FOS/JUN AP-1 complex to its cognate binding site. AP-endodeoxyribonuclease activity is not affected by CK2-mediated phosphorylation. Phosphorylation of Thr-233 by CDK5 reduces AP-endodeoxyribonuclease activity resulting in accumulation of DNA damage and contributing to neuronal death.
Cellular Location: Nucleus. Nucleus > Nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm.

Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm.
UniProt: P27695
Gene ID: 328
Synonyms: AP endonuclease 1 Antibody
AP endonuclease class I Antibody
AP lyase Antibody
APE Antibody
APE 1 Antibody
APE-1 Antibody
APE1 Antibody
APEN Antibody
APEX Antibody
APEX 1 Antibody
APEX nuclease Antibody
APEX nuclease (multifunctional DNA repair enzyme) 1 Antibody
Apex nuclease 1 Antibody
APEX1_HUMAN Antibody
Apurinic endonuclease Antibody
Apurinic-apyrimidinic endonuclease 1 Antibody
Apurinic/apyrimidinic (abasic) endonuclease Antibody
Apurinic/apyrimidinic endonuclease 1 Antibody
Apurinic/apyrimidinic exonuclease Antibody
APX Antibody
BAP1 Antibody
Deoxyribonuclease (apurinic or apyrimidinic) Antibody
DNA (apurinic or apyrimidinic site) lyase Antibody
DNA-(apurinic or apyrimidinic site) lyase, mitochondrial Antibody
EC 4.2.99.18 Antibody
HAP 1 Antibody
HAP1 Antibody
Human Apurinic endonuclease 1 Antibody
MGC139790 Antibody
Multifunctional DNA repair enzyme Antibody
Redox factor 1 Antibody
Redox factor-1 Antibody
REF 1 Antibody
REF 1 protein Antibody
REF-1 Antibody
REF1 Antibody
REF1 protein Antibody

Images
Western Blot - Anti-APEX1 Antibody from ABclonal (A0476) - Antibody Supply Service
Western blot analysis of MCF-7 cell lysate using APEX1 antibody.

Product Code - A0476
Size Price Quantity
100µl £243
200µl £372

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