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Anti-PRKCD Antibody

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ABclonal
A0471
Overview
Name: Anti-PRKCD Antibody
Description: Rabbit polyclonal antibody to PRKCD.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:100
Reactivity: Human
Immunogen: Recombinant protein of human PRKCD
Protein Length: 676
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion (By similarity). Phosphorylates ELAVL1 in response to angiotensin-2 treatment (PubMed:18285462).
Involvement in Disease: Autoimmune lymphoproliferative syndrome 3: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. CVID9 patients have B-cell deficiency and severe autoimmunity.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Post-Translational Modification: Autophosphorylated and/or phosphorylated at Thr-507, within the activation loop; phosphorylation at Thr-507 is not a prerequisite for enzymatic activity. Autophosphorylated at Ser-299, Ser-302 and Ser-304. Upon TNFSF10/TRAIL treatment, phosphorylated at Tyr-155; phosphorylation is required for its translocation to the endoplasmic reticulum and cleavage by caspase-3. Phosphorylated at Tyr-313, Tyr-334 and Tyr-567; phosphorylation of Tyr-313 and Tyr-567 following thrombin stimulation potentiates its kinase activity. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2.
Cellular Location: Cytoplasm. Cytoplasm > Perinuclear region. Nucleus. Cell membrane.
UniProt: Q05655
Gene ID: 5580
Synonyms: ALPS3 Antibody
CVID9 Antibody
D14Ertd420e Antibody
Kinase PKC delta Antibody
KPCA_HUMAN Antibody
KPCD Antibody
KPCD_HUMAN Antibody
MAY 1 Antibody
MAY1 Antibody
MGC49908 Antibody
nPKC delta Antibody
nPKC-delta Antibody
nPKC-theta Antibody
PCKd Antibody
PKC Antibody
PKC (phospho T410) Antibody
PKC (phospho T497) Antibody
PKC alpha Antibody
PKC beta Antibody
PKC d Antibody
PKC delta Antibody
PKC delta (phospho S299) Antibody
PKC delta (phospho S645) Antibody
PKC delta (phospho S664) Antibody
PKC delta (phospho T505) Antibody
PKC delta (phospho T507) Antibody
PKC delta (phospho Y311) Antibody
PKC delta (phospho Y313) Antibody
PKC delta (phospho Y52) Antibody
PKC delta (phospho Y64) Antibody
PKC delta + PKC theta Antibody
PKC epsilon Antibody
PKC gamma Antibody
PKC zeta Antibody
PKC-A Antibody
PKC-alpha Antibody
PKC2 Antibody
PKCA Antibody
PKCB Antibody
PKCD Antibody
PKCdelta Antibody
PKCE Antibody
PKCG Antibody
PRKC D Antibody
PRKC delta Antibody
PRKCA Antibody
PRKCB Antibody
PRKCE Antibody
PRKCG Antibody
PRKCQ Antibody
PRKCT Antibody
PRKCZ Antibody
Protein kinase C alpha Antibody
Protein kinase C alpha type Antibody
Protein kinase C beta Antibody
Protein kinase C delta Antibody
Protein kinase C delta type Antibody
Protein kinase C delta VIII Antibody
Protein kinase C epsilon Antibody
Protein kinase C gamma Antibody
Protein kinase C theta type Antibody
Protein kinase C zeta Antibody
Protein Kinase Cdelta Antibody
SDK1 Antibody
Sphingosine-dependent protein kinase-1 Antibody
Tyrosine protein kinase PRKCD Antibody
Tyrosine-protein kinase PRKCD Antibody

Images
Western Blot - Anti-PRKCD Antibody from ABclonal (A0471) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using PRKCD antibody.
Immunohistochemistry - Anti-PRKCD Antibody from ABclonal (A0471) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human liver cancer using PRKCD antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-PRKCD Antibody from ABclonal (A0471) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human esophagus using PRKCD antibody at dilution of 1:100 (40x lens).

Product Code - A0471
Size Price Quantity
50µl £148
100µl £243
200µl £372

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