phone icon
+44 (0)1223 298875

Anti-p70 S6 Kinase Antibody

file icon Datasheet
ABclonal
A0446
Overview
Name: Anti-p70 S6 Kinase Antibody
Description: Rabbit polyclonal antibody to p70 S6 Kinase.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:100
Reactivity: Human
Immunogen: A synthetic peptide of human RPS6KB1
Protein Length: 525
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.
Tissue Specificity: Widely expressed.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Post-Translational Modification: Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Cellular Location: Cell junction > Synapse > Synaptosome. Mitochondrion outer membrane. Mitochondrion.

Colocalizes with URI1 at mitochondrion.
UniProt: P23443
Gene ID: 6198
Synonyms: 70 kDa ribosomal protein S6 kinase 1 Antibody
KS6B1_HUMAN Antibody
p70 alpha Antibody
P70 beta 1 Antibody
p70 ribosomal S6 kinase alpha Antibody
p70 ribosomal S6 kinase beta 1 Antibody
p70 S6 kinase alpha Antibody
p70 S6 kinase, alpha 1 Antibody
p70 S6 kinase, alpha 2 Antibody
p70 S6K Antibody
p70 S6K-alpha Antibody
p70 S6KA Antibody
p70(S6K) alpha Antibody
p70(S6K)-alpha Antibody
p70-alpha Antibody
p70-S6K Antibody
p70-S6K 1 Antibody
P70S6K Antibody
P70S6K1 Antibody
p70s6kA Antibody
p70S6Kb Antibody
PS6K Antibody
Ribosomal protein S6 kinase 70kDa polypeptide 1 Antibody
Ribosomal protein S6 kinase beta 1 Antibody
Ribosomal protein S6 kinase beta-1 Antibody
Ribosomal protein S6 kinase I Antibody
Ribosomal protein S6 kinase, 70-kD Antibody
RPS6KB1 Antibody
S6K Antibody
S6K-beta-1 Antibody
S6K1 Antibody
S6K1 (phospho S235 + S236) Antibody
S6K1 (phospho S240) Antibody
S6K1 (phospho S411) Antibody
S6K1 (phospho S418) Antibody
S6K1 (phospho S424) Antibody
S6K1 (phospho T229) Antibody
S6K1 (phospho T389) Antibody
S6K1 (phospho T412) Antibody
S6K1 (phospho T421) Antibody
S6K1 (phospho T449) Antibody
Serine/threonine kinase 14 alpha Antibody
Serine/threonine protein kinase 14A Antibody
Serine/threonine-protein kinase 14A Antibody
STK14A Antibody

Images
Western Blot - Anti-p70 S6 Kinase Antibody from ABclonal (A0446) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cells, using RPS6KB1 antibody.

Product Code - A0446
Size Price Quantity
100µl £243
200µl £372

or
Request a quotation
Contact us directly to order
  • call icon +44 (0)1223 298875
  • +44 (0)1223 281447
  • email icon orders@ab-y-ss.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe