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Anti-cdc25C Antibody

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ABclonal
A0444
Overview
Name: Anti-cdc25C Antibody
Description: Mouse monoclonal antibody to cdc25C.
Applications: WB
Dilutions: WB 1:500 - 1:1000
Reactivity: Human
Immunogen: A synthetic peptide of human CDC25C
Protein Length: 473
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Sequence Similarities: Belongs to the MPI phosphatase family.
Post-Translational Modification: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Cellular Location: Nucleus.
UniProt: P30307
Gene ID: 995
Synonyms: CDC 25 Antibody
Cdc 25C Antibody
CDC25 Antibody
Cdc25C (phospho S198) Antibody
Cdc25C (phospho S216) Antibody
Cdc25C (phospho T48) Antibody
Cell division cycle 25 homolog C Antibody
Cell division cycle 25C Antibody
Cell division cycle 25C protein Antibody
Dual specificity phosphatase Cdc25C Antibody
M phase inducer phosphatase 3 Antibody
M-phase inducer phosphatase 3 Antibody
Mitosis inducer CDC25 Antibody
MPIP3 Antibody
MPIP3_HUMAN Antibody
Phosphotyrosine phosphatase Antibody
PPP1R60 Antibody
protein phosphatase 1, regulatory subunit 60 Antibody

Images
Western Blot - Anti-cdc25C Antibody from ABclonal (A0444) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using CDC25C antibody.

Product Code - A0444
Size Price Quantity
100µl £243
200µl £372

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