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Anti-cdc25C Antibody

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ABclonal
A0444
Details
Description: Mouse monoclonal antibody to cdc25C.
Category: Primary Antibodies
Applications: WB
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Reactivity: Human
UniProt: P30307
Gene ID: 995
Immunogen: A synthetic peptide of human CDC25C
Protein Length: 473
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: cdc25C; CDC25C; CDC25
Purification: Affinity purification
Background: cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000

Target (Information from UniProt)
Function: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Sequence Similarities: Belongs to the MPI phosphatase family.
Post-Translational Modification: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Cellular Location: Nucleus.

Images
Western Blot - Anti-cdc25C Antibody from ABclonal (A0444) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using CDC25C antibody.

Product Code - A0444
Size Price Quantity
100µl £243
200µl £372

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