|Description:||Mouse monoclonal antibody to XRCC1.|
|Immunogen:||Recombinant protein of human XRCC1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||XRCC1; RCC; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1|
|Background:||The X-ray repair cross complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the XRCC1 gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8).|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000|
Target (Information from UniProt)
|Function:||Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.|
|Post-Translational Modification:||Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.|
Accumulates at sites of DNA damage.
Western blot analysis of extracts of various cells, using XRCC1 antibody.