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Anti-mTOR Antibody

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ABclonal
A0380
Overview
Name: Anti-mTOR Antibody
Description: Rabbit polyclonal antibody to mTOR.
Applications: WB, IHC, IF
Dilutions: WB 1:200 - 1:500, IHC 1:50 - 1:100, IF 1:50 - 1:200
Reactivity: Human
Immunogen: A synthetic peptide of human mTOR
Protein Length: 2549
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity).
Tissue Specificity: Expressed in numerous tissues, with highest levels in testis.
Involvement in Disease: Smith-Kingsmore syndrome: An autosomal dominant syndrome characterized by intellectual disability, macrocephaly, seizures, umbilical hernia, and facial dysmorphic features.
Sequence Similarities: Belongs to the PI3/PI4-kinase family.
Post-Translational Modification: Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.
Cellular Location: Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Microsome membrane.

Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137).
UniProt: P42345
Gene ID: 2475
Synonyms: dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) Antibody
FK506 binding protein 12 rapamycin associated protein 1 Antibody
FK506 binding protein 12 rapamycin associated protein 2 Antibody
FK506 binding protein 12 rapamycin complex associated protein 1 Antibody
FK506-binding protein 12-rapamycin complex-associated protein 1 Antibody
FKBP rapamycin associated protein Antibody
FKBP12 rapamycin complex associated protein Antibody
FKBP12-rapamycin complex-associated protein Antibody
FKBP12-rapamycin complex-associated protein 1 Antibody
FLJ44809 Antibody
FRAP Antibody
FRAP1 Antibody
FRAP2 Antibody
Mammalian target of rapamycin Antibody
Mechanistic target of rapamycin Antibody
mTOR (phospho S2448) Antibody
mTOR (phospho S2481) Antibody
mTOR (phospho T2446) Antibody
MTOR_HUMAN Antibody
OTTHUMP00000001983 Antibody
RAFT1 Antibody
Rapamycin and FKBP12 target 1 Antibody
Rapamycin associated protein FRAP2 Antibody
Rapamycin target protein Antibody
Rapamycin target protein 1 Antibody
RAPT1 Antibody
Serine/threonine-protein kinase mTOR Antibody

Images
Western Blot - Anti-mTOR Antibody from ABclonal (A0380) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using MTOR antibody.

Product Code - A0380
Size Price Quantity
100µl £243
200µl £372

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