|Description:||Rabbit polyclonal antibody to GSTP1.|
|Applications:||WB, IHC, IF, FC|
|Immunogen:||A synthetic peptide of human GSTP1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||GSTP1; DFN7; FAEES3; GST3; PI|
|Background:||Glutathione S-transferases (GSTs) are a family of isoenzymes that detoxify electrophiles through conjugation to thiol-reduced glutathione (GSH). Thus, they are critical in protecting cells from toxins (drugs, pesticides, carcinogens) and oxidative stress (1). Eight isoforms of cytosolic-soluble GSTs (α, κ, μ, π, σ, θ, ζ, and ω) are identified, while only GST-α, -μ, and -π are described in the central nervous system (2). GSTP1 (GSTπ) is overexpressed in early stages of carcinogenesis and can be used as a neoplastic marker in tumor tissues (3). GSTP1 directly inhibits TRAF2 and JNK but not NF-κB (4,5). Corresponding GSTP1 gene polymorphisms affect substrate selectivity and stability, and the oxidative milieu in dopaminergic neurons, which increases the susceptibility to Parkinson’s disease (6).|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:1000, IHC 1:20 - 1:50, IF 1:20 - 1:50, FC 1:20 - 1:50|
Target (Information from UniProt)
|Function:||Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.|
|Sequence Similarities:||Belongs to the GST superfamily. Pi family.|
|Cellular Location:||Cytoplasm. Mitochondrion. Nucleus.
The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.
Western blot analysis of extracts of various cell lines, using GSTP1 antibody.