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Anti-CD40LG Antibody

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ABclonal
A0327
Details
Description: Rabbit polyclonal antibody to CD40LG.
Category: Primary Antibodies
Applications: WB, IHC, IF
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P29965
Gene ID: 959
Immunogen: Recombinant protein of human CD40LG
Protein Length: 261
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: CD40LG; CD154; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39; hCD40L
Purification: Affinity purification
Background: CD40 Ligand (CD40L), a member of the TNF superfamily of ligands, is expressed as either a membrane-bound or soluble homotrimer (1). Both membrane-bound and soluble forms of CD40L have biological activity (1,2). CD40L is expressed primarily on activated T cells; however, mast cells, basophils, and NK cells may also express CD40L (1). CD40L functions as an important T cell co-stimulatory molecule and enhances T-dependent B cell responses (1,2). CD40L binds CD40, which is a receptor expressed on B cells, dendritic cells, and macrophages (1). Binding of CD40L to CD40 leads to the recruitment of TRAF proteins (TRAFs 2,3,5, and 6) resulting in the activation of the JNK and NF-κB pathways (1,2).
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200

Target (Information from UniProt)
Function: Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).
Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes.
Involvement in Disease: X-linked immunodeficiency with hyper-IgM 1: Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence Similarities: Belongs to the tumor necrosis factor family.
Post-Translational Modification: The soluble form derives from the membrane form by proteolytic processing.
Cellular Location: Cell membrane. Cell surface.

Images
Western Blot - Anti-CD40LG Antibody from ABclonal (A0327) - Antibody Supply Service
Western blot analysis of extracts of various cells, using CD40LG antibody.
Immunofluorescence - Anti-CD40LG Antibody from ABclonal (A0327) - Antibody Supply Service
Immunofluorescence analysis of U20S cell using CD40LG antibody. Blue: DAPI for nuclear staining.

Product Code - A0327
Size Price Quantity
50µl £148
100µl £243
200µl £372

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