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Anti-UHRF1 Antibody

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Name: Anti-UHRF1 Antibody
Description: Rabbit polyclonal antibody to UHRF1.
Applications: WB, IF, FC
Dilutions: WB 1:500 - 1:2000, IF 1:20 - 1:50, FC 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human UHRF1
Protein Length: 793
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Tissue Specificity: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Post-Translational Modification: Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome.
Cellular Location: Nucleus.

Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
UniProt: Q96T88
Gene ID: 29128
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Western Blot - Anti-UHRF1 Antibody from ABclonal (A0297) - Antibody Supply Service
Western blot analysis of extracts of MCF7 cell line, using UHRF1 antibody.

Product Code - A0297
Size Price Quantity
100µl £243
200µl £372

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