Anti-Hexokinase II AntibodyDatasheet
|Description:||Rabbit polyclonal antibody to Hexokinase II.|
|Immunogen:||A synthetic peptide of human HK2|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||Hexokinase II; HK2; DKFZp686M1669; HKII; HXK2|
|Background:||Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:1000, IHC 1:50 - 1:100|
Target (Information from UniProt)
|Tissue Specificity:||Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.|
|Sequence Similarities:||Belongs to the hexokinase family.|
|Cellular Location:||Mitochondrion outer membrane.
Its hydrophobic N-terminal sequence may be involved in membrane binding.
Western blot analysis of extracts of HeLa cells, using HK2 antibody.