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Anti-ISG15 Antibody

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ABclonal
A0154
Overview
Name: Anti-ISG15 Antibody
Description: Rabbit polyclonal antibody to ISG15.
Applications: WB, IHC
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200
Reactivity: Human
Immunogen: A synthetic peptide of human ISG15
Protein Length: 165
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.
Tissue Specificity: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.
Involvement in Disease: Immunodeficiency 38, with basal ganglia calcification: A primary immunodeficiency predisposing individuals to severe clinical disease upon infection with weakly virulent mycobacteria, including Mycobacterium bovis Bacille Calmette-Guerin (BCG) vaccines. Patients are also susceptible to Salmonella and Mycobacterium tubercolosis infections. Affected individuals have intracranial calcification.
Post-Translational Modification: S-nitrosylation decreases its dimerization, thereby increasing the availability as well as the solubility of monomeric ISG15 for its conjugation to cellular proteins.
Cellular Location: Cytoplasm. Secreted.

Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins.
UniProt: P05161
Gene ID: 9636
Synonyms: G1P2 Antibody
hUCRP Antibody
IFI 15 Antibody
IFI15 Antibody
Interferon alpha inducible protein Antibody
Interferon induced protein 15 Antibody
Interferon induced protein IFI-15K Antibody
Interferon stimulated protein 15 kDa Antibody
Interferon, alpha-inducible protein (clone IFI-15K) Antibody
Interferon-induced 15 kDa protein Antibody
Interferon-induced 17 kDa protein Antibody
Interferon-induced 17-kDa/15-kDa protein Antibody
IP17 Antibody
ISG 15 Antibody
ISG15 ubiquitin like modifier Antibody
ISG15_HUMAN Antibody
Ubiquitin cross-reactive protein Antibody
Ubiquitin-like protein ISG15 Antibody
UCRP Antibody

Images
Western Blot - Anti-ISG15 Antibody from ABclonal (A0154) - Antibody Supply Service
Western blot analysis of extracts of HeLa cell line, using ISG15 antibody.

Product Code - A0154
Size Price Quantity
100µl £243
200µl £372

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