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Anti-FEN-1 Antibody

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ABclonal
A0129
Overview
Name: Anti-FEN-1 Antibody
Description: Rabbit polyclonal antibody to FEN-1.
Applications: WB, IF
Dilutions: WB 1:500 - 1:1000, IF 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human FEN1
Protein Length: 380
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Sequence Similarities: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Post-Translational Modification: Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Cellular Location: Nucleus > Nucleolus. Nucleus > Nucleoplasm.

Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
UniProt: P39748
Gene ID: 2237
Synonyms: DNase IV Antibody
FEN1 Antibody
FEN1_HUMAN Antibody
Flap endonuclease 1 Antibody
Flap structure specific endonuclease 1 Antibody
Flap structure-specific endonuclease 1 Antibody
hFEN-1 Antibody
hFEN1 Antibody
Maturation factor 1 Antibody
MF1 Antibody
Rad2 Antibody

Images
Western Blot - Anti-FEN-1 Antibody from ABclonal (A0129) - Antibody Supply Service
Western blot analysis of extracts of HepG2 cells, using FEN1 antibody.

Product Code - A0129
Size Price Quantity
100µl £243
200µl £372

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