|Description:||Rabbit polyclonal antibody to FEN-1.|
|Dilutions:||WB 1:500 - 1:1000, IF 1:20 - 1:50|
|Immunogen:||A synthetic peptide of human FEN1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.|
|Sequence Similarities:||Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.|
|Post-Translational Modification:||Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.|
|Cellular Location:||Nucleus > Nucleolus. Nucleus > Nucleoplasm.
Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
|Synonyms:||DNase IV Antibody
Flap endonuclease 1 Antibody
Flap structure specific endonuclease 1 Antibody
Flap structure-specific endonuclease 1 Antibody
Maturation factor 1 Antibody
Western blot analysis of extracts of HepG2 cells, using FEN1 antibody.