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Anti-Cyclin H Antibody

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Description: Rabbit polyclonal antibody to Cyclin H.
Category: Primary Antibodies
Applications: WB, FC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P51946
Gene ID: 902
Immunogen: A synthetic peptide of human CCNH
Protein Length: 323
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: CCNH; CAK; p34; p37
Purification: Affinity purification
Background: CCNH belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression (1,2). In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). CCNH is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36/Mat1, which enhances binding between CCNH and CDK7 and increases activity (3,4). CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes (5,6). The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity (7,8,9). When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II (9), providing a link between cell cycle control, transcriptional regulation, and DNA repair.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, FC 1:20 - 1:50

Target (Information from UniProt)
Function: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Sequence Similarities: Belongs to the cyclin family. Cyclin C subfamily.
Cellular Location: Nucleus.

Western Blot - Anti-Cyclin H Antibody from ABclonal (A0107) - Antibody Supply Service
Western blot analysis of extracts of HeLa cells, using CCNH antibody.

Product Code - A0107
Size Price Quantity
100µl £243
200µl £372

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