|Description:||Rabbit polyclonal antibody to Ubc9.|
|Immunogen:||A synthetic peptide of human UBE2I|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||P18; UBC9; C358B7.1|
|Background:||The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:1000, IHC 1:50 - 1:100|
Target (Information from UniProt)
|Function:||Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.|
|Tissue Specificity:||Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.|
|Sequence Similarities:||Belongs to the ubiquitin-conjugating enzyme family.|
|Post-Translational Modification:||Phosphorylation at Ser-71 significantly enhances SUMOylation activity.|
|Cellular Location:||Nucleus. Cytoplasm.
Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Western blot analysis of extracts of A2058 cells, using UBE2I antibody.