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Anti-Ubc9 Antibody

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ABclonal
A0105
Details
Description: Rabbit polyclonal antibody to Ubc9.
Category: Primary Antibodies
Applications: WB, IHC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P63279
Gene ID: 7329
Immunogen: A synthetic peptide of human UBE2I
Protein Length: 158
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: P18; UBC9; C358B7.1
Purification: Affinity purification
Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:100

Target (Information from UniProt)
Function: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Tissue Specificity: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family.
Post-Translational Modification: Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Cellular Location: Nucleus. Cytoplasm.

Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).

Images
Western Blot - Anti-Ubc9 Antibody from ABclonal (A0105) - Antibody Supply Service
Western blot analysis of extracts of A2058 cells, using UBE2I antibody.

Product Code - A0105
Size Price Quantity
100µl £243
200µl £372

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