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Anti-Pyk2 Antibody

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ABclonal
A0074
Overview
Name: Anti-Pyk2 Antibody
Description: Rabbit polyclonal antibody to Pyk2.
Applications: WB, IHC, IF
Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:50
Reactivity: Human
Immunogen: A synthetic peptide of human PTK2B
Protein Length: 3030
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purification: Affinity purification
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at 4℃. Avoid freeze / thaw cycles.

Target (Information from UniProt)
Function: Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Tissue Specificity: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Post-Translational Modification: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.
Cellular Location: Cytoplasm. Cytoplasm > Perinuclear region. Cell membrane. Cell junction > Focal adhesion. Cell projection > Lamellipodium. Cytoplasm > Cell cortex. Nucleus.

Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.
UniProt: Q14289
Gene ID: 2185
Synonyms: CADTK Antibody
CAK-beta Antibody
CAKB Antibody
CAKbeta Antibody
Calcium regulated non receptor proline rich tyrosine kinase Antibody
Calcium-dependent tyrosine kinase Antibody
Cell adhesion kinase beta Antibody
E430023O05Rik Antibody
EC 2.7.10.2 Antibody
FADK 2 Antibody
FADK2 Antibody
FAK2 Antibody
FAK2_HUMAN Antibody
Focal adhesion kinase 2 Antibody
MGC124628 Antibody
PKB Antibody
Proline-rich tyrosine kinase 2 Antibody
Protein kinase B Antibody
Protein Tyrosine Kinase 2 Beta Antibody
Protein-tyrosine kinase 2-beta Antibody
PTK Antibody
PTK2B Antibody
PTK2B protein tyrosine kinase 2 beta Antibody
PYK2 (phospho Y402) Antibody
PYK2 (phospho Y579 + Y580) Antibody
PYK2 (phospho Y579) Antibody
PYK2 (phospho Y580) Antibody
PYK2 (phospho Y881) Antibody
RAFTK Antibody
RAFTK2 Antibody
Related adhesion focal tyrosine kinase Antibody

Images
Western Blot - Anti-Pyk2 Antibody from ABclonal (A0074) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cell line, using PTK2B antibody.

Product Code - A0074
Size Price Quantity
100µl £243
200µl £372

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