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Anti-HNRNPC Antibody

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ABclonal
A0057
Details
Description: Rabbit polyclonal antibody to HNRNPC.
Category: Primary Antibodies
Applications: WB, IHC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P07910
Gene ID: 3183
Immunogen: A synthetic peptide of human HNRNPC
Protein Length: 306
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: C1; C2; HNRNP; HNRPC; SNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
Purification: Affinity purification
Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) are predominantly nuclear RNA-binding proteins that form complexes with RNA polymerase II transcripts. These proteins function in a variety of cellular activities, ranging from transcription and pre-mRNA processing in the nucleus to cytoplasmic mRNA translation and turnover. hnRNP C1/C2 is one of the most abundant proteins in the nucleus, and shown to have roles in cellular differentiation and proliferation. A novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from the nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells. Overexpression of hnRNP C1/C2 in hepatocellular carcinoma (HCC) tissues has been reported in 70 HCC samples, suggesting its potential as a protein tumor marker.
Storage: Store at 4℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200

Target (Information from UniProt)
Function: Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).
Sequence Similarities: Belongs to the RRM HNRPC family. RALY subfamily.
Post-Translational Modification: Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Cellular Location: Nucleus.

Component of ribonucleosomes.

Images
Western Blot - Anti-HNRNPC Antibody from ABclonal (A0057) - Antibody Supply Service
Western blot analysis of extracts of various cell lines, using HNRNPC antibody.

Product Code - A0057
Size Price Quantity
100µl £243
200µl £372

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