|Description:||Rabbit polyclonal antibody to HNRNPC.|
|Immunogen:||A synthetic peptide of human HNRNPC|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||C1; C2; HNRNP; HNRPC; SNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody|
|Background:||Heterogeneous nuclear ribonucleoproteins (hnRNPs) are predominantly nuclear RNA-binding proteins that form complexes with RNA polymerase II transcripts. These proteins function in a variety of cellular activities, ranging from transcription and pre-mRNA processing in the nucleus to cytoplasmic mRNA translation and turnover. hnRNP C1/C2 is one of the most abundant proteins in the nucleus, and shown to have roles in cellular differentiation and proliferation. A novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from the nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells. Overexpression of hnRNP C1/C2 in hepatocellular carcinoma (HCC) tissues has been reported in 70 HCC samples, suggesting its potential as a protein tumor marker.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200|
Target (Information from UniProt)
|Function:||Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).|
|Sequence Similarities:||Belongs to the RRM HNRPC family. RALY subfamily.|
|Post-Translational Modification:||Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.|
Component of ribonucleosomes.
Western blot analysis of extracts of various cell lines, using HNRNPC antibody.