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Anti-MGMT Antibody

file icon Datasheet
Description: Rabbit polyclonal antibody to MGMT.
Category: Primary Antibodies
Applications: WB
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human
UniProt: P16455
Gene ID: 4255
Immunogen: Recombinant Protein of human MGMT
Protein Length: 207
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Background: MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:2000

Target (Information from UniProt)
Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Sequence Similarities: Belongs to the MGMT family.
Cellular Location: Nucleus.

Western Blot - Anti-MGMT Antibody from ABclonal (A0052) - Antibody Supply Service
Western blot analysis of extracts of Jurkat cell line, using MGMT antibody.

Product Code - A0052
Size Price Quantity
50µl £148
100µl £243
200µl £372

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