|Description:||Rabbit polyclonal antibody to BRAF.|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:200|
|Immunogen:||Recombinant protein of human BRAF|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.|
|Tissue Specificity:||Brain and testis.|
|Involvement in Disease:||Colorectal cancer: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.
Lung cancer: A common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
Familial non-Hodgkin lymphoma: Cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Cardiofaciocutaneous syndrome 1: A multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices.
Noonan syndrome 7: A form of Noonan syndrome, a disease characterized by short stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits, multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of myelomonocytic cells.
LEOPARD syndrome 3: A disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
|Sequence Similarities:||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.|
|Post-Translational Modification:||Phosphorylation at Ser-365 by SGK1 inhibits its activity.|
|Cellular Location:||Nucleus. Cytoplasm. Cell membrane.
Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
|Synonyms:|| FLJ95109 Antibody
94 kDa B raf protein Antibody
B Raf Antibody
B Raf (mutated V600 E) Antibody
B Raf (phospho S446) Antibody
B Raf (phospho S601) Antibody
B Raf (phospho S729) Antibody
B Raf (phospho T401) Antibody
B Raf (phospho T598 + S601) Antibody
B Raf (phospho T598) Antibody
B Raf (phospho T599) Antibody
B Raf (phospho T753) Antibody
B raf 1 Antibody
B Raf proto oncogene serine threonine protein kinase Antibody
B Raf proto oncogene, serine/threonine kinase Antibody
B RAF1 Antibody
B-Raf proto-oncogene serine/threonine-protein kinase (p94) Antibody
BRAF 1 Antibody
Murine sarcoma viral (v-raf) oncogene homolog B1 Antibody
Murine sarcoma viral v raf oncogene homolog B1 Antibody
Oncogen BRAF Antibody
oncogene BRAF1 Antibody
Proto-oncogene B-Raf Antibody
Proto-oncogene c-Rmil Antibody
RAFB 1 Antibody
Serine/threonine-protein kinase B-raf Antibody
Sm protein E Antibody
Small nuclear ribonucleoprotein E Antibody
v raf murine sarcoma viral oncogene homolog B Antibody
v raf murine sarcoma viral oncogene homolog B1 Antibody
v-Raf murine sarcoma viral oncogene homolog B1 Antibody
Western blot analysis of extracts of various cells, using BRAF antibody.