Anti-Cyclin A AntibodyDatasheet
|Name:||Anti-Cyclin A Antibody|
|Description:||Rabbit polyclonal antibody to Cyclin A.|
|Applications:||WB, IHC, FC|
|Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:100, FC 1:20 - 1:50|
|Immunogen:||A synthetic peptide of human CCNA2|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at 4℃. Avoid freeze / thaw cycles.|
Target (Information from UniProt)
|Function:||Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.|
|Sequence Similarities:||Belongs to the cyclin family. Cyclin AB subfamily.|
|Post-Translational Modification:||Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.|
|Cellular Location:||Nucleus. Cytoplasm.
Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).
Western blot analysis of extracts of K-562 cell line, using CCNA2 antibody.