Anti-Podoplanin Antibody (Biotin)
|Description:||Rabbit polyclonal antibody to Podoplanin (Biotin)|
|Applications:||ELISA, IHC, IP, WB|
|Reactivity:||Human, Monkey, Mouse|
|Concentration:||0.55-0.75 µg/µl in antibody stabilization buffer|
|Background:||Invovled in cell migration and actin cytoskeleton organization. Induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion when expressed in keratinocytes.|
|Storage:||-20⁰C for long term storage|
|Recommended Dilutions:||DB: 1:10,000; ELISA: 1:10,000; Western Blot: 1:500|
Target (Information from UniProt)
|Function:||May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.|
|Tissue Specificity:||Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.|
|Sequence Similarities:||Belongs to the podoplanin family.|
|Post-Translational Modification:||Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.|
|Cellular Location:||Membrane. Cell projection > Filopodium membrane. Cell projection > Lamellipodium membrane. Cell projection > Microvillus membrane. Cell projection > Ruffle membrane.
Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.