Anti-Phospho-Slingshot homolog 1 Antibody (Biotin)
|Name:||Anti-Phospho-Slingshot homolog 1 Antibody (Biotin)|
|Description:||Rabbit polyclonal antibody to Phospho-Slingshot homolog 1 (Biotin)|
|Dilutions:||DB: 1:10,000; ELISA: 1:10,000; Western Blot: 1:500|
|Reactivity:||Human, Monkey, Mouse, Rat|
|Immunogen:||Phosphorylated synthetic peptide corresponding to unique amino acid sequence on Slingshot homolog 1 protein.|
|Concentration:||0.64-0.72 µg/µl in antibody stabilization buffer|
|Storage:||-20⁰C for long term storage|
Target (Information from UniProt)
|Function:||Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.|
|Sequence Similarities:||Belongs to the protein-tyrosine phosphatase family.|
|Post-Translational Modification:||Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.|
|Cellular Location:||Cytoplasm > Cytoskeleton. Cell projection > Lamellipodium. Cleavage furrow. Midbody.
Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.