|Description:||Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)|
|Applications:||WB, ICC/IF, IHC|
|Reactivity:||Human, Horse, Cow, Pig, Chicken, Rat, Mouse|
|Immunogen:||Native GFAP purified from bovine spinal cord.|
|Concentration:||Antibody is supplied as an aliquot of concentrated IgY preparation.|
|Introduction:||Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and coworkers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kDa protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander’s disease was recently shown to be caused by point mutations in the protein coding region of the GFAP gene (2). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. The antibody was made against a recombinant construct of human GFAP protein. This antibody has been used in several recent publications (see 4). The HGNC name for this protein is GFAP.|
|Molecular Weight:||~55 kDa|
|Storage:||Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB: 1:10,000. IF/ICC and IHC: 1:1,000-1:5,000.|
Mixed cultures of neurons and glia stained with CPCA-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
Western blot of whole rat brain (lane1) and mouse brain (lane2) homogenate stained with CPCA-GFAP, at dilution of 1:5,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to GFAP. Also shown is protein size marker in red. Note, molecular weight of mouse GFAP is smaller than that of rat GFAP.