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Anti-GFAP Antibody

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EnCor Biotechnology
CPCA-GFAP
Overview
Name: Anti-GFAP Antibody
Description: Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)
Applications: WB, ICC/IF, IHC
Recommended Dilutions: WB: 1:10,000. IF/ICC and IHC: 1:1,000-1:5,000.
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse
Immunogen: Native GFAP purified from bovine spinal cord.
Host: Chicken
Clonality: Polyclonal
Isotype: IgY
Concentration: Antibody is supplied as an aliquot of concentrated IgY preparation.
Molecular Weight: ~55 kDa
Storage: Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Target
Introduction: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and coworkers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kDa protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander’s disease was recently shown to be caused by point mutations in the protein coding region of the GFAP gene (2). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. The antibody was made against a recombinant construct of human GFAP protein. This antibody has been used in several recent publications (see 4). The HGNC name for this protein is GFAP.
Synonyms: wu:fb34h11 Antibody
ALXDRD Antibody
cb345 Antibody
etID36982.3 Antibody
FLJ42474 Antibody
FLJ45472 Antibody
GFAP (phospho S38) Antibody
GFAP (phospho S8) Antibody
GFAP delta Antibody
GFAP Epsilon Antibody
GFAPdelta Antibody
GFAPepsilon Antibody
gfapl Antibody
GFAP_HUMAN Antibody
Glial fibrillary acidic protein Antibody
Intermediate filament protein Antibody
wu:fk42c12 Antibody
xx:af506734 Antibody
zgc:110485 Antibody

Images
Immunohistochemisty - Anti-GFAP Antibody from EnCor Biotechnology (CPCA-GFAP) - Antibody Supply Service
Mixed cultures of neurons and glia stained with CPCA-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
Western Blot - Anti-GFAP Antibody from EnCor Biotechnology (CPCA-GFAP) - Antibody Supply Service
Western blot of whole rat brain (lane1) and mouse brain (lane2) homogenate  stained with CPCA-GFAP, at dilution of 1:5,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to GFAP. Also shown is protein size marker in red. Note, molecular weight of mouse GFAP is smaller than that of rat GFAP.

References

Liem RKH, Yen SH, Salomon GD and Shelanski ML. Intermediate filaments in nervous tissues. J Cell Biol 79:637-745 (1978).

Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972.

Bruijnzeel AW, Bauzo RM, Munikoti V, Rodrick GB, Yamada H, Fornal CA, Ormerod BK, Jacobs BL.Tobacco smoke diminishes neurogenesis and promotes gliogenesis in the dentate gyrus of adolescent rats. Brain Res. 1413:32-42 (2011).

Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 2001


Product Code - CPCA-GFAP
Size Price Quantity
50µl £165
100µl £230
500µl £714

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