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Anti-S162 phospho-MAPRE3 (EB3) Antibody

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Absea Biotechnology
Name: Anti-S162 phospho-MAPRE3 (EB3) Antibody
Description: Mouse monoclonal (KT114) antibody to S162 phospho-MAPRE3 (EB3).
Applications: WB, IHC
Reactivity: Human
Immunogen: S162 phosphorylated EB3 peptide conjugated to carrier protein.
Amino Acid Start: 177
Amino Acid End: 420
Host: Mouse
Clonality: Monoclonal
Clone: KT114
Isotype: IgG2b
Conjugate: Unconjugated
Purification: Antibody is purified from culture supernatant using a protein A column.
Concentration: 1 mg/ml in PBS containing 0.09% sodium azide as a preservative.
Storage: 4 °C for 1 month, -20 °C or -80°C in aliquots. Avoid repeated freeze/thaw cycles.

Target (Information from UniProt)
Function: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).
Tissue Specificity: Predominantly expressed in brain and muscle.
Sequence Similarities: Belongs to the MAPRE family.
Cellular Location: Cytoplasm > Cytoskeleton.

Associated with the microtubule network. Detected at the plus end of microtubules.
UniProt: Q9UPY8
Gene ID: 22924

Immunohistochemistry - Anti-S162 phospho-MAPRE3 (EB3) Antibody from Absea Biotechnology (163404B07) - Antibody Supply Service
Rat immunoglobulin isotyping by ELISA using rat primary antibodies and HRP conjugated KT96, KT97, KT98 and KT99 as secondary antibodies.
Western Blot - Anti-S162 phospho-MAPRE3 (EB3) Antibody from Absea Biotechnology (163404B07) - Antibody Supply Service
Western blotting using lysates of HEK cells expressing wild type EB3 (WT, most S162 was phosphorylated) and none phosphorylated EB3 (S162A). WT EB3 and S162A EB3 were expressed as fusion proteins with GFP. The bands were detected using KT114 and anti-GFP antibodies. Courtesy of Dr. Yulia Komarova.

Product Code - 163404B07
Size Price Quantity
100µg £186
200µg £273
1mg £730

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