Anti-S162 phospho-MAPRE3 (EB3) AntibodyDatasheet
|Name:||Anti-S162 phospho-MAPRE3 (EB3) Antibody|
|Description:||Mouse monoclonal (KT114) antibody to S162 phospho-MAPRE3 (EB3).|
|Immunogen:||S162 phosphorylated EB3 peptide conjugated to carrier protein.|
|Amino Acid Start:||177|
|Amino Acid End:||420|
|Purification:||Antibody is purified from culture supernatant using a protein A column.|
|Concentration:||1 mg/ml in PBS containing 0.09% sodium azide as a preservative.|
|Storage:||4 °C for 1 month, -20 °C or -80°C in aliquots. Avoid repeated freeze/thaw cycles.|
Target (Information from UniProt)
|Function:||Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).|
|Tissue Specificity:||Predominantly expressed in brain and muscle.|
|Sequence Similarities:||Belongs to the MAPRE family.|
|Cellular Location:||Cytoplasm > Cytoskeleton.
Associated with the microtubule network. Detected at the plus end of microtubules.
Rat immunoglobulin isotyping by ELISA using rat primary antibodies and HRP conjugated KT96, KT97, KT98 and KT99 as secondary antibodies.
Western blotting using lysates of HEK cells expressing wild type EB3 (WT, most S162 was phosphorylated) and none phosphorylated EB3 (S162A). WT EB3 and S162A EB3 were expressed as fusion proteins with GFP. The bands were detected using KT114 and anti-GFP antibodies. Courtesy of Dr. Yulia Komarova.