|Description:||Rabbit polyclonal antibody to SNAI1.|
|Reactivity:||Human, Mouse, Rat|
|Immunogen:||A synthetic peptide of human SNAI1|
|Formulation:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonyms:||SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1|
|Background:||The Drosophila embryonic protein SNAI1 is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
|Recommended Dilutions:||WB 1:500 - 1:2000, IHC 1:50 - 1:100|
Target (Information from UniProt)
|Function:||Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.|
|Tissue Specificity:||Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.|
|Sequence Similarities:||Belongs to the snail C2H2-type zinc-finger protein family.|
|Post-Translational Modification:||Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.|
|Cellular Location:||Nucleus. Cytoplasm.
Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Immunohistochemistry of paraffin-embedded human colon carcinoma using SNAI1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human esophagus using SNAI1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SNAI1 antibody at dilution of 1:100 (x40 lens).