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Anti-HAVCR2 Antibody

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ABclonal
A2516
Details
Description: Rabbit polyclonal antibody to HAVCR2.
Category: Primary Antibodies
Applications: WB, IHC
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Reactivity: Human, Mouse, Rat
UniProt: Q8TDQ0
Gene ID: 84868
Immunogen: A synthetic peptide of human HAVCR2
Protein Length: 301
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonyms: TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Purification: Affinity purification
Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Recommended Dilutions: WB 1:500 - 1:1000, IHC 1:50 - 1:200

Target (Information from UniProt)
Function: Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock (By similarity).
Tissue Specificity: Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.
Sequence Similarities: Belongs to the immunoglobulin superfamily. TIM family.
Post-Translational Modification: O-glycosylated with core 1 or possibly core 8 glycans.
Cellular Location: Membrane. Cell junction.

Localizes to the immunological synapse between CD8+ T-cells and target cells.

Images
Western Blot - Anti-HAVCR2 Antibody from ABclonal (A2516) - Antibody Supply Service
Western blot analysis of extracts of various cells, using HAVCR2 antibody.
Western Blot - Anti-HAVCR2 Antibody from ABclonal (A2516) - Antibody Supply Service
Western blot analysis of extracts of various cells, using HAVCR2 antibody.
Immunohistochemistry - Anti-HAVCR2 Antibody from ABclonal (A2516) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human lung cancer using HAVCR2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry - Anti-HAVCR2 Antibody from ABclonal (A2516) - Antibody Supply Service
Immunohistochemistry of paraffin-embedded human lung cancer using HAVCR2 antibody at dilution of 1:100 (x40 lens).

Product Code - A2516
Size Price Quantity
50µl £148
100µl £243
200µl £372

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