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Anti-CLASP2 Antibody

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Absea Biotechnology
032006E03
Overview
Name: Anti-CLASP2 Antibody
Description: Rat monoclonal (KT68) antibody to CLASP2.
Applications: WB, IF 
Reactivity: Human
Immunogen: Recombinant CLASP2 (90 - 876 bp in KIAA0627).
Amino Acid Start: 177
Amino Acid End: 420
Host: Rat
Clonality: Monoclonal
Clone: KT68
Isotype: IgG2b
Conjugate: Unconjugated
Purification: Antibody is purified from culture supernatant using a protein G column.
Concentration: 1 mg/ml in PBS containing 0.09% sodium azide as a preservative.
Storage: 4 °C for 1 month, -20 °C or -80°C in aliquots. Avoid repeated freeze/thaw cycles.

Target (Information from UniProt)
Function: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
Tissue Specificity: Brain-specific.
Sequence Similarities: Belongs to the CLASP family.
Post-Translational Modification: Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding (PubMed:26003921). Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.
Cellular Location: Cytoplasm > Cytoskeleton. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Chromosome > Centromere > Kinetochore. Cytoplasm > Cytoskeleton > Spindle. Golgi apparatus. Golgi apparatus > trans-Golgi network. Cell membrane. Cell projection > Ruffle membrane.

Localizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.
UniProt: O75122
Gene ID: 23122
Synonyms: CLAP2_HUMAN Antibody
CLASP 2 Antibody
CLIP associated protein 2 Antibody
CLIP associating protein 2 Antibody
CLIP-associating protein 2 Antibody
Cytoplasmic linker associated protein 2 Antibody
Cytoplasmic linker-associated protein 2 Antibody
hOrbit2 Antibody
KIAA0627 Antibody
Multiple asters (Mast) like homolog 2 Antibody
Protein Orbit homolog 2 Antibody

Product Citations

Maffini S et al. Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux. Curr. Biol. 19(18): 1566-1572 (2009)


Product Code - 032006E03
Size Price Quantity
100µg £186
200µg £273
1mg £730

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